IL-18 and perforin deficiency alter CD8 T-cell RICD and proliferation. (A) RICD in CD8 T cells in the presence (purple) or absence (blue) of 50 ng/uL IL-18 after a 24-hour restimulation with CD3/CD28. Each data point represents the average of technical triplicates within 1 experiment. P values are determined using two-way ANOVA. (B) Percent and representative graphs of in vivo EdU incorporation in CD8 T cells 19h after systemic administration. Data from >3 separate experiments; each data point represents a single mouse. P values calculated by one-way ANOVA with Tukey multiple comparisons test, evaluating all comparisons with DS^KO group. Only adjusted P < .05 is shown.