STAT1 is required for HAPLN1 matrikine–induced MM cell migration and BM homing. (A) Graphs depicting the percentage migration of MM.1S shControl or shSTAT1 clones in response to MBP or MBP-PTR1, with MBP-treated shControl being set as 100%. (B) The tibia-to-femur ratio of PKH26⁺ MM percentage was plotted as in Figure 2B. (C) Graph depicting the normalized expression of genes in the red cluster. Data are expressed as mean ± SD. (D) The dot plot represents the enrichment scores of the top 30 pathways in which the size of dot indicates the number of genes enriched for each pathway, and the color intensity correlates with the adjusted P. See “Methods” for enrichment score calculations. (E-F) mRNA levels of indicated genes was quantified using qRT-PCR and normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and fold change relative to control (MBP in wild-type [WT] cells) were plotted. The graph represents the means ± SEM of 5 biological replicates for panel E and 3 biological replicates for panel F, each performed in duplicates. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001.