Loss of FOXO3 improves RBC production in β-thalassemia. (A) FOXO3 ablation alters BM erythroblast frequencies. ∗P < .05 between WT and Hbb^th3/+ groups and ^#P < .05 between Hbb^th3/+ and Foxo3^−/−/Hbb^th3/+ groups (n ≥ 3). (B) Fold change of annexin V⁺ cells in live Foxo3^−/−, Hbb^th3/+, and Foxo3^−/−/Hbb^th3/+ relative to WT BM TER119⁺ cells in each gate. ∗P < .05, ∗∗P < .01. (C) Macroscopic examination of the spleen from WT, Hbb^th3/+, and Foxo3^−/−/Hbb^th3/+. Results are shown as mean ± SD.