Timing and location of donor cell engraftment in the brain. (A) Myeloablative transplantation schema. (B) Flow cytometry gating strategy for bone marrow cell populations. (C) Chimerism of GFP⁺ donor cells in recipient PBMC. (D-E) Chimerism of GFP⁺ donor cells in the recipient hematogenic cell populations. (F-H) Sagittal brain sections at 7 and 30 days after HCT. Red outlines define various brain regions. Engrafted GFP⁺ donor cells are demarcated by colored circles. Scale bars represent 500 μm in panels F and G, and 200 μm in panel H. (I) Enumeration of donor cells according to brain regions as the percentage of the total number for cells counted. (J) Enumeration of donor cells normalization to area of brain region counted. Shown are the mean ± standard deviation. BMMC, bone marrow mononuclear cells; CC, corpus collosum; FSC, forward scatter; LSK cells, Lin⁻SCA1⁺cKIT⁺ cells; PBMC, peripheral blood mononuclear cells; SSC, side scatter.