GSK3$β$deletion in hematopoietic cells does not promote oncogenic transformation. (A) No differences in serial colony formation are observed using GSK3β WT and KO bone marrow cells with or without the expression of MLL-AF9 (n = 3 per condition). (B) No differences in the frequency of white or red blood cells in primary recipient mouse were observed in the peripheral blood after bone marrow transplant. CBC ± SEM for recipient mice 13 weeks after transplantation (n = 6 for GSK3β KO and n = 7 for each control). (C) No differences in the spleen morphology or mass were observed among mice that underwent primary engraftment. Representative bone spleen sections from recipient mice after receiving 2 × 10⁶ GSK3β WT or KO bone marrow transplant cells 13 weeks after transplant (n = 5 per group). Sections were stained with H&E, and images were captured at original magnification ×4 (n = 4 per condition). (D) No differences in the frequency of HPCs among mice that underwent primary engraftment were observed in the peripheral blood after bone marrow transplant. Average frequency of hematopoietic cell populations from murine bone marrow as measured by flow cytometry (n = 5). (E) No differences in the frequency of white or red blood cells in secondary engraftment recipient mouse were observed in the peripheral blood after bone marrow transplant. CBC ± SEM for secondary recipient mice 13 weeks after transplantation (n = 6 for GSK3β KO, n = 6 for control). (F) No differences in spleen morphology or mass were observed among mice that underwent secondary engraftment. Representative spleen sections from recipient mice after receiving 2 × 10⁶ GSK3β WT or KO bone marrow transplant cells 13 weeks after transplant (n = 5). Sections were stained with H&E, and images were captured at original magnification ×4 (n = 4 per condition). (G) No differences in the frequency of HPCs among mice that underwent secondary engraftment were observed in the peripheral blood after bone marrow transplant. Average frequency of hematopoietic cell populations from murine bone marrow as measured by flow cytometry (n = 5 per group).