Expression levels of PTEN and its regulating miRNAs measured by qRT-PCR before and after treatment with alpelisib. (A) PTEN expression levels in PBMCs. PTEN expression was normalized to HPRT1 endogenous controls. (B) miR-21-5p, miR-26a-5p, and miR-181c-5p expression in PBMCs and (C) plasma samples. MiRNA expression was normalized to U6 endogenous control and spike-in control cel-miR-39, respectively. The histograms represent the relative expression from at least 3 experiments. ∗P < .05; ∗∗P < .001; ∗∗∗P < .0001. HPRT1, hypoxanthine phosphoribosyltransferase 1.