Characterization of Treg subsets by scRNA-seq. Volcano plots based on the scRNA-seq data from healthy donor tonsils (n = 3), FL (n = 3), and DLBCL (n = 3), showing key significantly DEGs between (A) activated and resting Tregs and (B) activated and LAG3⁺ Tregs. Each dot indicates 1 gene; red dots represent a log₂ fold change (log₂FC) of >0.35, and blue dots depict a log₂FC < −0.35. Significant genes were selected with adjusted P value < .05. Full list of DEGs is shown in supplemental Tables 6 and 7. (C) Dot plot of selected genes from scRNA-seq data, showing average gene expression of checkpoint receptors, transcription factors, cytokines, and DEGs across the 3 distinct cellular subsets of Tregs. (D) Heat map of gene set enrichment analysis (GSEA) highlighting highly enriched pathways among the 3 Treg clusters based of Hallmark and KEGG (K) libraries, shown as enrichment score.