Significant transcriptomic changes associated with hemostasis in the liver of macaques infected with LASV Josiah, LASV AV, and MACV. (A) Transcriptomic analysis of liver cells at 2, 5 or 11 DPI for AV and Josiah or at time of death for MACV. The heatmaps represent the gene expression in each pathway. Only statistically differentially expressed genes (for Josiah vs mock, Josiah vs AV, or MACV vs mock comparisons) are included. The color of the heatmaps represents the mock–related scaled normalized counts averaged for each group and time point (n = 3 for each time point and infection status and n = 6 for MACV). Gene names were annotated regarding their differential expression status: asterisk refers to genes significant in the mock-AV-Josiah data set whereas degree symbols refer to genes significant in the Machupo data set. (B) Violin plots comparing the global expression variation relative to the mock. The overall gene sets over- or under-expression was tested with a 1-way mixed analysis of variance and P-values were corrected with the Tukey multiple comparison test.