Figure 4.
Cdc73 is important for genome integrity. (A) Western blot for γH2AX in Rosa26CreERT2Cdc73f/f T-ALL cells (969 and 970) and control Rosa26CreERT2 T-ALL cells treated with OHT for 30 hours to delete Cdc73. Numbers represent band intensities normalized to β-actin loading control. (B) Western blot for γH2AX in human NOTCH1-induced human T-ALL cell lines (SUP-T1; CUTLL1) transduced with shCDC73. (C-E) Representative metaphase spreads (C) and quantification of metaphase abnormalities in aggregate (D) or per replicate (E) in blinded analyses of Cdc73f/f T-ALL cells treated with OHT for 30 hours. White arrows represent gaps; orange arrow represents break. (F-G) Representative Annexin V/7-AAD flow cytometric plots (F) and Annexin V+/7-AAD− scatterplot (G) of Cdc73f/f T-ALL cells treated with vehicle (Control) or OHT for 30 hours (Cdc73Δ/Δ). 7-AAD, 7-aminoactinomycin D.

Cdc73 is important for genome integrity. (A) Western blot for γH2AX in Rosa26CreERT2Cdc73f/f T-ALL cells (969 and 970) and control Rosa26CreERT2 T-ALL cells treated with OHT for 30 hours to delete Cdc73. Numbers represent band intensities normalized to β-actin loading control. (B) Western blot for γH2AX in human NOTCH1-induced human T-ALL cell lines (SUP-T1; CUTLL1) transduced with shCDC73. (C-E) Representative metaphase spreads (C) and quantification of metaphase abnormalities in aggregate (D) or per replicate (E) in blinded analyses of Cdc73f/f T-ALL cells treated with OHT for 30 hours. White arrows represent gaps; orange arrow represents break. (F-G) Representative Annexin V/7-AAD flow cytometric plots (F) and Annexin V+/7-AAD− scatterplot (G) of Cdc73f/f T-ALL cells treated with vehicle (Control) or OHT for 30 hours (Cdc73Δ/Δ). 7-AAD, 7-aminoactinomycin D.

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