GLS1 expression in human DLBCL cell lines and biopsy samples. (A) GLS1, GLS2, and c-MYC protein expression in various ABC and GCB DLBCL cell lines was analyzed by immunoblotting. GAPDH served as loading control. Note that the protein doublet of the GLS1 blot might correspond to different GLS1 isoforms. (B) Immunohistochemical staining of GLS1 in human ABC and GCB DLBCL cell lines. Scale bars, 50 μm. (C) Immunohistochemical staining of GLS1 in human DLBCL biopsies (left pictures). The cytoplasmic GLS1 expression was quantified in DLBCL biopsy samples (n = 42, right panel).