CB-839 induces cytotoxicity in DLBCL. Various ABC DLBCL cell lines (A), GCB DLBCL cell lines (B), or human primary B cells (C) were treated with solvent or 400 nM CB-839 and incubated for the indicated time. Cell numbers were determined daily and normalized to the solvent control. Error bars correspond to the mean ± standard deviation (SD). Data are representative of 3 for panels A-B or 2 for panel C independent experiments. (A-B) Statistical significance was calculated using the Student t test (unpaired, 2-tailed) to compare the solvent with the CB-839–treated samples at day 6.