Glutaminolysis inhibition and glutamine deprivation impair DLBCL survival. Various ABC DLBCL cell lines (A) or GCB DLBCL cell lines (B) were treated with solvent or 1 μM BPTES. After the indicated time, cell numbers were determined and normalized to the solvent control. (C) DOHH2 and HBL-1 cells were transduced with either a nontargeting vector control or an short hairpin RNA (shRNA) targeting GLS. After transduction, cell numbers were determined daily as indicated and normalized to the nontargeting control. (D) The efficacy of the shRNA-mediated knockdown of GLS1 in DOHH2 and HBL-1 cells was controlled by immunoblotting. Tubulin served as a loading control. (E) ABC and GCB DLBCL cell lines were cultured in standard or glutamine-deprived medium for 5 days. Cell numbers were determined and normalized to the standard medium control. Error bars correspond to the mean ± SD. Data are representative of 3 for panels A-B or 2 for panels C-E independent experiments. (A-B) Statistical significance was calculated using the Student t test (unpaired, 2-tailed) to compare the solvent with the BPTES-treated samples at day 6.