Figure 3.
Gata2 germline variation prevents polyvinyl alcohol-supported HSPC expansion ex vivo. (A) Schematic for isolation and culture of LT-HSCs in defined medium.34 (B) Representative flow cytometric plots for FACS-isolation of LT-HSCs (LSK CD150+ CD48− CD34–). (C) Flow cytometric quantification of total live cells and LT-HSCs (LSK CD150+ CD48− CD34–) per well. N = 3 replicates per condition, average of 3 experiments. ∗P < .05, ∗∗P < .01, ∗∗∗P < .001; 1-way ANOVA followed by Tukey multiple comparisons test. DAPI, 4′,6-diamidino-2-phenylindole.

Gata2 germline variation prevents polyvinyl alcohol-supported HSPC expansion ex vivo. (A) Schematic for isolation and culture of LT-HSCs in defined medium.34 (B) Representative flow cytometric plots for FACS-isolation of LT-HSCs (LSK CD150+ CD48− CD34–). (C) Flow cytometric quantification of total live cells and LT-HSCs (LSK CD150+ CD48− CD34–) per well. N = 3 replicates per condition, average of 3 experiments. ∗P < .05, ∗∗P < .01, ∗∗∗P < .001; 1-way ANOVA followed by Tukey multiple comparisons test. DAPI, 4′,6-diamidino-2-phenylindole.

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