Gata2 germline variation selectively attenuates acute HSPC mobilization. (A) Mice were treated with 5 mg/kg plerixafor (CXCR4 antagonist), 1 mg/kg rhIL-8 (CXCR2 agonist), or 1 mg/kg BIO5192 (VLA-4 antagonist) and harvested after 0.25, 1, or 2 hours. (B) CFU from 0.1 mL of peripheral blood. (C) Peripheral blood neutrophil quantification. N = 3 to 12 per condition, 9 experiments. ∗P < .05, ∗∗P < .01, ∗∗∗P < .001; 1-way ANOVA followed by Tukey multiple comparisons test. PBS, phosphate-buffered saline.