CPX induces intracellular iron deprivation that mediates the HSC supportive effect. (A) Numbers of CD34⁺CD90⁺CD71⁺ cells after 6 days culture (n = 3). (B) Calcein-AM staining in CD34⁺ cells treated with CPX or DMSO for 1 hour (n = 3). (C) Quantification of CD34⁺CD90⁺ cell number after 6 days culture with DMSO, CPX, or ferric amonium citrate (FAC) (n = 3). (D) Pathway analysis of altered metabolites from CB-CD34⁺ cells treated with CPX or DMSO, the orange line represents the cutoff of significance (P = .05). (E-F) OCR measured by Seahorse assay on CB-CD34⁺ cells.