GPRC5C induces the expression of SLC7A5 and SLC43A1 via NF-κB signaling. (A) Experimental scheme to overexpress GPRC5C in KG1. (B) qPCR of SLC7A5 and SLC43A1 expression after GPRC5C-OE in KG1. Normalized to housekeeping gene GAPDH/ACTB and KG1-control; n = 5. (C) Mean fluorescence intensity measurement of pNF-κB in KG1-control and KG1- GPRC5C -OE cells with FACS plot representation; n = 10. (D) Experimental scheme of IKKi inhibition to prevent NF-κB activation in KG1-Control and KG1-GPRC5C-OE cells. (E) qPCR of SLC7A5 and SLC43A1 expression after GPRC5C -OE in KG1 with or without treatment with IKK inhibitor. Normalized to housekeeping gene GAPDH/ACTB and KG1-control; n = 5. (F) GSEA of the LSC signature from Eppert et al in KG1-control and KG1- GPRC5C-OE cells with or without treatment with IKK inhibitor (BMS-345541). GSEA was performed with BH-adjusted P values after the adaptive multilevel splitting Monte Carlo approach. All represented by mean ± standard deviation. Unpaired Student t test was performed unless otherwise indicated ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗ P < 0.0001. For all experiments, at least 2 independent experiments were performed. BH, Benjamini-Hochberg correction; DMSO, dimethyl sulfoxide; MFI, mean fluorescence intensity; IKKi, IκB kinase inhibitor.