Identification of very short FLT3 insertions in a multicenter cohort of patients with AML. (A) Flowchart identifying patients with very short FLT3 insertions within the GBMHM network. (B) The genomic localization of the 20 FLT3-VSIs with accessible sequencing data is presented in the context of their respective protein and exon sequences. The term #FLT3 mutations indicates the number of FLT3-VSIs identified at specific amino acid (aa) positions. The number of FLT3-VSIs within the domains spanning aa 572 to 578 and 591 to 599 is given. (C) The genomic landscape of FLT3-VSI in 22 patients from our cohort is shown. The results were obtained during routine diagnostic procedures performed by clinical laboratories at the time of AML diagnosis. Only variants identified at least twice within the cohort are presented in this oncoprint. FLT3-ITD∗ indicates additional ITD mutations. (D) Survival curves for the entire cohort of patients with FLT3-VSI (n = 18 with available data).