Fc-mediated complement activation is associated with macrophage trafficking from the lungs to the blood in in vivo antibody-mediated TRALI. BALB/c WT (A-E) and C5-deficient (F-G) mice were primed as in Figures 1 and 2, followed by infusion of indicated antibodies. (A) Blood, (B) lung tissue, and (C) BALF of BALB/c WT mice treated with respective antibodies 4.5 mg/kg IV, and (D) blood and (E) lung tissue of BALB/c WT mice treated with the indicated human chimeric antibodies, 1 mg/kg IV. (F) Blood and (G) lung tissue of C5-deficient mice (C57BL/6 with H2-k^d) treated with mouse IgG2a antibodies (4.5 mg/kg IV). Only statistical differences of interest are shown; each dot represents 1 mouse. Data are shown as means ± SD; n = 4 to 6; ∗P < .05; ∗∗P < .01; ∗∗∗P < .001. Statistical analysis was performed with a one-way ANOVA with a Tukey post hoc test.