NETs are induced in an in vitro TRALI setting with C5a, which mimics the complement-inducing effect of 34-1-2S. (A) Neutrophils from the bone marrow of C57BL/6 WT mice in a chemotaxis assay under indicated conditions. (B) In vitro NET formation under indicated conditions. (C) Representative images; original magnification ×20‍, with digital zoom to increase visibility. Only statistical differences of interest are shown; each dot represents neutrophils isolated from 1 individual mouse, n = 3 to 4. Data are shown as means ± SD. Statistical analysis was performed with a one-way ANOVA with a Tukey post hoc test; ∗P < .05; ∗∗P <.01.