Blocking the C5aR does not prevent antibody-mediated murine TRALI. C57BL/6 WT mice were primed as described earlier (Figure 1), followed by IV infusion of both 34-1-2S (45 mg/kg) and AF6-88.5.5.3 (4.5 mg/kg) together with diluent control or the small chemical C5aR antagonist, DF2593A (10 mg/kg). (A) Lung W/D weight ratio at 90 minutes; (B) rectal temperatures; and (C) macrophage levels in the blood and (D) lung at 90 minutes, n = 6. All dots represent individual mice. All mice survived until the 90-minute time point. Data are shown as means ± SD. Statistical analyses was performed with a 1-tailed unpaired t test; ∗P < .05. CTRL, control.