The count of PSLPs increases during storage of apheresis PLTs at RT. (A) PSLP counts measured by Coulter counter for 5 individual apheresis PLT products. (B) FC scatter plots of PSLPs at days 1, 3, 5, and 7 of storage. (C) Representative FC size distribution plot of PSLPs on day 5 storage (red histogram represents silica standard size particles). FC-based counts of small (D) and large (E) PSLPs up to day 7 of storage (N = 12). FC data are expressed as the fold change in the count of small and large PEVs normalized to that of the day 1 platelet bag (mean ± SE) (¤P < .001; #P < .01; ∗P < .05; N = 12). (F) Nanoparticle tracking analysis (NTA) of PSLPs (N = 5). (G) Representative NTA histogram shows the broad size distribution of PSLPs.
Figure 2.

The count of PSLPs increases during storage of apheresis PLTs at RT. (A) PSLP counts measured by Coulter counter for 5 individual apheresis PLT products. (B) FC scatter plots of PSLPs at days 1, 3, 5, and 7 of storage. (C) Representative FC size distribution plot of PSLPs on day 5 storage (red histogram represents silica standard size particles). FC-based counts of small (D) and large (E) PSLPs up to day 7 of storage (N = 12). FC data are expressed as the fold change in the count of small and large PEVs normalized to that of the day 1 platelet bag (mean ± SE) (¤P < .001; #P < .01; ∗P < .05; N = 12). (F) Nanoparticle tracking analysis (NTA) of PSLPs (N = 5). (G) Representative NTA histogram shows the broad size distribution of PSLPs.

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