American Society of Hematology

Figure 5.

$FC analysis of PSLP populations isolated by density gradient. Distribution and scatter plots of (A) PEV ghosts, (B) large/dense PEVs, and (C) DPLTs and large/dense PEVs. FC analysis of percentage of PS+CD62P+ PSLPs and PS+CD41a+ PSLPs present in the PSLP populations isolated by density gradient (D). N = 3, t test; P < .05 considered significant. TG assay demonstrates the procoagulant activities of PSLP populations isolated by sucrose density gradient. TG curves for PSLPs in the absence (E) and presence of 10 μmol/L of lactadherin (F), representative of 3 individual experiments and 3 individual donors. The same volume (50 μL) of each PSLP fraction was used in the experiment. RFU, relative fluorescence units.$

FC analysis of PSLP populations isolated by density gradient. Distribution and scatter plots of (A) PEV ghosts, (B) large/dense PEVs, and (C) DPLTs and large/dense PEVs. FC analysis of percentage of PS+CD62P+ PSLPs and PS+CD41a+ PSLPs present in the PSLP populations isolated by density gradient (D). N = 3, t test; P < .05 considered significant. TG assay demonstrates the procoagulant activities of PSLP populations isolated by sucrose density gradient. TG curves for PSLPs in the absence (E) and presence of 10 μmol/L of lactadherin (F), representative of 3 individual experiments and 3 individual donors. The same volume (50 μL) of each PSLP fraction was used in the experiment. RFU, relative fluorescence units.

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