Apheresis PLTs release mitochondria (MITO) during storage at RT. TEM image of (A) extracellular MITO in apheresis PLT supernatant on day 7 of storage and (B) a mitochondrion in an autophagic vacuole. Red arrows indicate mitochondria. FC analysis of (C) PS⁺TOM20⁺TOM20⁺LC3⁺, DHPE⁺LC3⁺, and DHPE⁺TOM20⁺LC3⁺ PSLPs. There is an increase in PS⁺TOM20⁺ PSLPs with storage time. The counts of mitophagy-related PSLPs do not significantly change during storage. FC data are expressed as the fold change in the count of small and large PEVs normalized to that of from day 1 PLT bag (mean ± SE) (#P < .01; ∗P < .05; N = 12).