Validation of anti-GATA2 mAbs. (A) Anti-GATA2 western blots of whole-cell lysates from mouse G1E ER-GATA1 cells, mouse HoxB8-immortalized myeloid progenitor cells, and mouse HoxB8-immortalized myeloid progenitors with homozygous deletion of the −77 Gata2 enhancer element resulting in reduced GATA2 expression. (B) Anti-GATA2 western blots of K562 whole-cell lysate. (C) Anti-GATA2 IP using rabbit polyclonal anti-GATA2 antibody or rabbit immunoglobulin G (IgG) control, and western blot with 15D2 anti-GATA2 mAb.