Transcriptome analysis of aged CD61^High and CD61^Low LT-HSCs. (A) Schematic representation of the RNA-sequencing experiment (mice age, >22 months). (B) Sorting strategy for CD61^High and CD61^Low LT-HSCs. (C) MDS plot showing the principal coordinates (PC) analysis of CD61^High and CD61^Low LT-HSCs. (D) differentially expressed (DE) genes in aged CD61^High and CD61^Low LT-HSCs. Volcano plot showing distribution of the adjusted P value (−log₁₀P value) and the fold changes (FC; logFC). Upregulated and downregulated genes are indicated in red and blue, respectively (P-adj < .01). (E) Signature enrichment plot from gene set enrichment analysis (GSEA) using a low-output HSC gene set in LT-HSCs from CD61^High and CD61^Low cells (GSE134242). (F) Signature enrichment plot from GSEA using a dormant HSC gene set in LT-HSCs from CD61^High and CD61^Low cells (GSE87814). (G) GSEA for the most downregulated pathways in CD61^High LT-HSCs. NES, normalized enrichment score.