Inhibitor studies. (A) Schematic representation of inhibition of CD61 signaling with small molecules. (B) Histogram representing coexpression of CD51 or CD41 with CD61 in aged LT-HSCs. (C) FACS plot showing coexpression of CD51 and CD61 on LSKs and LT-HSCs. (D) Single-cell division assay of aged CD61^High and CD61^Low LT-HSCs treated with tirofiban or cycloRGDγK in StemSpan with 5% serum supplementation and cytokines. (E) Single-cell division assay of aged CD61^High and CD61^Low LT-HSCs treated with tirofiban or cycloRGDγK in serum-free StemSpan supplemented with cytokines. IL-11, interleukin-11; LSK, Lin⁻Sca1⁺c-Kit.