Transplantation of CD61 repressed LT-HSCs. (A) Schematic representation of the shRNA-mediated CD61 repression. (B) CD61 mRNA levels after shRNA-mediated downregulation of CD61 in aged LT-HSCs. (C) CD61 protein levels after CD61 downregulation, 5 days after transduction. (D) Competitive transplantation assay of aged LT-HSCs after shRNA-mediated CD61 downregulation (CD61^KD) compared with scrambled shRNA (SCR) control cells (mice per group, n = 4). Data from representative experiment are shown. (E) PB chimerism levels 20 weeks after transplantation. (F) Donor-derived T cells, B cells, and myeloid cells, 20 weeks after transplantation. (G) Donor-derived LT-HSC frequency in the BM of recipient mice 20 weeks after transplantation. (H) Competitive transplantation assay of young LT-HSCs after shRNA-mediated CD61 downregulation (CD61^KD) compared with SCR control cells (mice per group; n = 4). Data from representative experiment are shown.