Somatic mutation VAF changes over time and age. (A-C) Somatic mutation VAF changes across samples collected at different time points. Colored dots and lines show mutations in CL and leukemia genes; gray dots and lines represent other somatic mutations. (D) Correlation between patient’s age and percentage of stable somatic mutations (present in at least 2 time points with VAF > 0.01) in all somatic mutations. Dot size indicates the total somatic mutation number in this patient. BM, bone marrow; PB, peripheral blood.