FXIII-A₂ does not alter F13b transcription or translation in mouse liver. (A) RNA was isolated from F13a1^+/+, F13a1^–/–, and rFXIII-A₂–treated F13a1^–/– mouse livers and F13b transcript level was determined by RT-qPCR using primers spanning each indicated exon junction. Dots show median fold change ± IQR, N = 4-5 per group. No significant differences in transcript levels relative to F13a1^+/+ mice were detected by Kruskal-Wallis test with Dunn post hoc testing. (B) Schematic of polysome profiling protocol. (C) F13b transcript abundance in the monosome and polysome fractions isolated from F13a1^+/+, F13a1^–/–, and rFXIII-A₂–treated F13a1^–/– mouse livers, represented as a monosome/polysome ratio. Dots represent individual mice, bars show mean ± standard deviation. Groups were compared with 1-way ANOVA with Dunnett post hoc testing. ANOVA, analysis of variance; RT-qPCR, reverse transcriptase-quantitative polymerase chain reaction; ns, nonsignificant.