miR-24 expression increases in EBV-infected and EBNA2-transfected B-cell lymphoma cell lines. (A) TargetScanHuman 7.0 algorithm predicted 4 consequential miR-24 seed sequence pairing in the 3′ UTR of ICOSL mRNA. (B) miR-24 expression was evaluated by RT-qPCR in (Bi-ii) B- and P-converted RAMOS and BJAB cells; (Biii) EBNA2-negative U2932 EBVGFP cl.A and EBNA2-positive cl.B; (Biv) in EBNA2-negative P3HR1 and its isogenic EBNA2-positive JIJOYE cells; (Bv) in EREB2-5–containing estradiol-inducible EBNA2. Statistical significance of the differences was estimated through Dunnett multiple comparison in ordinary one-way analysis of variance (ANOVA) test. (Ci-ii) miR-24 expression in EBNA2-transfected B-lymphoma cell lines. RT-qPCR was performed to assess the fold change of miR-24 levels in (Ci) U2932 EBNA2-transfected vs vector alone cell line and (Cii) in BJABK3 in which EBNA2 was induced with β-estradiol and its corresponding parental cell line BJABK3. P values were calculated through 2-tailed unpaired t test; ∗P < .05, ∗∗P < .01, ∗∗∗P < .001. The fold change of miR-24 was calculated with the formula 2^−ΔΔct, relative to the housekeeping gene RNU6 in each sample.