Metabolic markers of the storage lesion are poor correlates to hemolytic propensity in the REDS RBC Omics recalled donor cohort. (A-C) 3D uMAPs are color coded as a function of storage (spontaneous), oxidative, or osmotic hemolysis in the REDS RBC Omics recalled donor cohort (z-axis represent storage day, with uMAP1 and 2 calculated via uMAP). (D-F) Volcano plots indicate metabolite associations to the same hemolysis parameters, with the x-axis indicating the Spearman-determined correlation between hemolysis and metabolite levels and the y-axis indicating the −log10 of the false discovery rate–corrected P values for such associations). (G) Line plots indicate the donors in the 5th and 95th percentile (red and blue lines) for a selected metabolic markers of the storage lesion across the 3 time points (storage day 10, 23, and 42) tested for each donor (n = 643).