No cytotoxicity of AbTCR-CSR against neutrophils from normal donors. (A) Neutrophils from HLA-A∗02:01–positive donor or (B) HLA-A∗02:01–negative donor were isolated using a human whole-blood neutrophil isolation kit (Miltenyi) and incubated with mock T cells, CSR only, or AbTCR-CSR clone no. 18 or no. 34 at an E: T ratio of 1:1, overnight. The cells were stained with CD15 (for neutrophils) or CD3 (AbTCR-CSR T cells). The percentage of CD15⁺CFSE⁺ cells after coculture are shown in panel C and the positive control cell line AML-14 is shown in panel D. Because AML-14 cells do not express CD15, CD33 was used for its marker. The data represents 1 set of results from 4 independent donors.