Figure 2.
BCR-activated B cells from patients with active cGVHD B cells produce increased levels of IL-6 in response to ex vivo TLR7 stimulation. B cells from patients who had received HSCT with active (n = 9) or no cGVHD (n =10) at the time of sample collection were isolated by negative magnetic selection from PBMCs. Cells from healthy donors are shown for reference. B cells were cultured (A) in the absence of stimulation, or after stimulation with (B) low-dose anti-IgM alone (BCR stimulation, 0.625 μg/mL), (C) R848 alone (TLR7 stimulation, 1 μg/mL), or (D) R848 + low-dose anti-IgM. Culture supernatants were harvested at 24 hours and analyzed for levels of IL-6 via ELISA. Data represent median ± range. ∗P < .05 between sample groups. No, no cGVHD; Act, active cGVHD; H, healthy donor.

BCR-activated B cells from patients with active cGVHD B cells produce increased levels of IL-6 in response to ex vivo TLR7 stimulation. B cells from patients who had received HSCT with active (n = 9) or no cGVHD (n =10) at the time of sample collection were isolated by negative magnetic selection from PBMCs. Cells from healthy donors are shown for reference. B cells were cultured (A) in the absence of stimulation, or after stimulation with (B) low-dose anti-IgM alone (BCR stimulation, 0.625 μg/mL), (C) R848 alone (TLR7 stimulation, 1 μg/mL), or (D) R848 + low-dose anti-IgM. Culture supernatants were harvested at 24 hours and analyzed for levels of IL-6 via ELISA. Data represent median ± range. ∗P < .05 between sample groups. No, no cGVHD; Act, active cGVHD; H, healthy donor.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal