Continual development of tissue-resident macrophages and T cells from HSPCs. (A-D) PCA of the relationship of the level of all individual barcode reads retrieved, normalized to the total numbers of reads, from tissue-resident macrophages (red), PB monos (green), and T cells (blue) from the barcoded animals at multiple time points after transplantation. Symbol size represents biopsy time points relative to transplantation, symbol shapes to different tissues, and ellipses correspond to 95% confidence intervals.