Kinin generation from recombinant HK/LK-Met³⁷⁹ and HK/LK-Lys³⁷⁹. For all reactions, the kininogen concentration was 200 nM. (A) Time courses of kinin formation for HK-Met³⁷⁹ (blue) or HK-Lys³⁷⁹ (red) incubated with 2 nM PKa and (B) LK-Met³⁷⁹ (light green), or LK-Lys³⁷⁹ (yellow) incubated with 50 nM PKa. (C) Kinin formed from HK-Met³⁷⁹ (blue) or HK-Lys³⁷⁹ (red); and (D) LK-Met³⁷⁹ (light green) or LK-Lys³⁷⁹ (yellow) incubated for 30 minutes with 40 nM plasmin (Plm), 2 nM kallikrein (PKa), 1000 nM α-thrombin (αIIa), 20 nM FIXa, 20 nM FXa, 30 nM FXIa (or 25 nM FXIIa); vehicle (Veh.) is Tris-buffered saline with no added enzyme. (E) Time courses of HK-Met³⁷⁹ (blue) or HK-Lys³⁷⁹ (red); and (F) LK-Met³⁷⁹ (light green) or LK-Lys³⁷⁹ (yellow) incubated with 40 nM plasmin. For all reactions kinin generation was determined by ELISA, and results shown are means ± 1 standard deviation for 2 runs.