Kinin generation in normal plasma supplemented with recombinant kininogens. (A) Normal plasma was supplemented with 500 μM captopril, 10 μM KV999272 (kallikrein inhibitor), 600 nM recombinant Glu-plasminogen, 100 nM HK-Met³⁷⁹ or HK-Lys³⁷⁹, or control. (B) As in panel A, except the added kininogen was 100 nM LK-Met³⁷⁹ or LK-Lys³⁷⁹. In both sets of reactions, tPA was added to 125 nM to generate plasmin, and aliquots were taken at indicated time points for measurement of kinin concentration by ELISA. Shown are averages ± 1 standard deviation for 2 runs.