UBTF is localized to HOXA/HOXB regions in UBTF-TD AML but not in normal hematopoietic progenitors. (A) Genomic occupancy of UBTF-WT and UBTF-TD in cbCD34+ cells. UBTF occupancy was assessed via HA:CUT&RUN in cbCD34+ cells expressing HA-UBTF-WT (blue) or HA:UBTF-TD at days 10 (D10) for UBTF-WT and UBTF-TD, as well as day 32 (D32) and day 45 (D45) for UBTF-TD. (B) IGV tracks of UBTF at the rDNA, HOXB, and HOXA genomic loci of normal cbCD34+ cells from 3 biological replicates (blue), 2 different UBTF-TD PDX (colored in red), a KMT2A-r PDX (KMT2A::MLLT3, colored in black), and an MPAL PDX (black). (C) Rank ordered peak scores of UBTF peaks subset to promoters or rDNA in normal cbCD34+ cells, UBTF-TD PDX from B (UBTF-TD PDX–1), and the second UBTF-TD PDX (UBTF-TD PDX–2). Peaks at HOXA/HOXB (red) or rDNA (blue) are annotated.