A genome-wide CRISPR-Cas9 knockout screen identified the cBAF complex as an essential factor for leukemic cell migration toward CXCL12. (A) Screening strategy to identify genes essential for leukemic cell migration toward CXCL12. (B) Volcano plot showing the gRNA ratio (dropped/input) vs P value obtained from MAGeCK analysis. Depleted and nondepleted genes coding SWI/SNF subunits are highlighted in red and black, respectively. CXCR4, highlighted in blue, is a positive control. (C) Subunits of cBAF, PBAF, and ncBAF. Subunits identified as screening hits are highlighted in orange. (D-E) Migration activity of Jurkat cells in which the indicated genes are knocked out individually (D) or in combination (E). Cells expressing gAAVS1 were used as a control. Data are shown as mean ± SD (n = 3). (F) Migration activity of BRM014- or AU-15330–treated cells. Cells were treated at 1 μM for 3 days and then used for migration assays. Data are normalized to those of DMSO-treated cells and are shown as mean ± SD (n = 3). Two-tailed Student t test was used to assess statistical significance in panels D, E, and F (∗∗∗P < .001; ∗∗P < .01; ∗P < .05; n.s., not significant). DMSO, dimethyl sulfoxide; gRNA, guide RNA; MAGeCK, model-based analysis of genome-wide CRISPR-Cas9 knockout; PBAF, polybromo-associated BAF; SD, standard deviation.