The cBAF complex is required for the growth of human T-ALL in vivo. (A) Quantification of luminescence signals of mice transplanted with luciferase-labeled Jurkat cells in which the indicated genes were knocked out. Data are shown as mean (n = 3). (B-C) Percentage (B) and number (C) of human CD45⁺ cells in the bone marrow of mice transplanted with the indicated knockout Jurkat cells at day 28 after transplantation. Data are shown as mean ± SD (n = 3). (D-E) Ratio of BFP⁺ cells to mCherry⁺ cells in the bone marrow of mice transplanted with a 1:1 mixture of BFP⁺ indicated knockout Jurkat cells and mCherry⁺ control Jurkat cells at day 21 after transplantation. Data are normalized to those of the AAVS1 knockout cells and are shown as mean ± SD (n = 3) in panel E. (F) Treatment schedule for the Jurkat xenograft model and PDX models. Mice were euthanized 8 hours after the last treatment and used for the subsequent analyses (data shown in panels G-L). (G-H) Percentage (G) and number (H) of human CD45⁺ cells in the bone marrow of vehicle- and BRM014-treated xenograft models. Data are shown as mean ± SD (n = 3-4). (I-J) Cell surface CXCR4 expression on human CD45⁺ cells in the bone marrow of vehicle or BRM014-treated xenograft models. Data are shown as mean ± SD (n = 3-4) in J. (K-L) Relative gene expression of CXCR4 (K) and CDK6 (L) in human CD45⁺ cells in the bone marrow of vehicle- and BRM014-treated xenograft models, as determined by RT-qPCR. Data are shown as mean ± SD (n = 3-4). Two-tailed Student t test was used to assess statistical significance in panels B, C, E, G, H, J, K, and L (∗∗∗P < .001; ∗∗P < .01; ∗P < .05). BFP, blue fluorescent protein; RT-qPCR, reverse transcription quantitative polymerase chain reaction; SD, standard deviation.