Bulk RNA sequencing of E11.5 embryos reveals significant differences in genome expression. (A) Principal component analysis displays the difference in global gene expression profiles between wt/wt and mt/mt embryos. N = 5 for each genotype. (B) Clustering analysis of differentially expressed genes of A shown as a heat map. (C) RT-qPCR of angiogenesis- and platelet-related differentially expressed genes scored by bulk RNA-seq. RNA was extracted from E11.5 embryonic heads. N = 3 for each genotype. All data were analyzed using t-tests.