Figure 5.
Homozygotic P735R mutation of Gne causes hyposialylation in mice. (A) Western blotting of GNE and GAPDH as loading control using mouse tissue extracts at E10.5. (B) Confocal images of PNA staining in wt/wt and mt/mt E10.5 embryos. (C) Lectin blotting of E10.5 tissue extracts probed with MAL-II, which detects α2,3-sialylated glycans, SNA, which detects α2,6-sialylated glycans, and PNA, which detects non-sialylated core 1 O-glycans (also known as T-antigen). The blots are representative of 4 independent experiments. (D) Confocal images of MAL-II staining in wt/wt and mt/mt E10.5 embryos. MAL-II, Maackia amurensis lectin II; PNA, peanut agglutinin. N = 3 for each genotype in (B) and (D). Scale bar, 100 μm.

Homozygotic P735R mutation of Gne causes hyposialylation in mice. (A) Western blotting of GNE and GAPDH as loading control using mouse tissue extracts at E10.5. (B) Confocal images of PNA staining in wt/wt and mt/mt E10.5 embryos. (C) Lectin blotting of E10.5 tissue extracts probed with MAL-II, which detects α2,3-sialylated glycans, SNA, which detects α2,6-sialylated glycans, and PNA, which detects non-sialylated core 1 O-glycans (also known as T-antigen). The blots are representative of 4 independent experiments. (D) Confocal images of MAL-II staining in wt/wt and mt/mt E10.5 embryos. MAL-II, Maackia amurensis lectin II; PNA, peanut agglutinin. N = 3 for each genotype in (B) and (D). Scale bar, 100 μm.

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