T-cell phenotype in patients with HGBL-MYC/BCL2, patients with DLBCL NOS, and HDs. (A-B) Expression analysis of major T-cell subsets (A), and CD127 (B) in HDs (green; n = 16), patients with HGBL-MYC/BCL2 (red; n = 66), and patients with DLBCL NOS (blue; n = 53). (C) In-depth analysis of the computationally identified T-cell populations by FlowSOM expressed higher (red) or lower (blue) in patients with HGBL-MYC/BCL2 vs patients with DLBCL NOS. (D-E) Expression analysis of HLA-DR and CD38 (D) and PD-1 (E) in HDs (green; n = 16), patients with HGBL-MYC/BCL2 (red; n = 66), and patients with DLBCL NOS (blue; n = 53). (F) Dimensionality reduction by UMAP of the flow cytometry data of T-cells. Color overlays for CD38, HLA-DR, PD-1, and CD8 are depicted. For all box plots, the lower upper hinges correspond to the 25th and 75th percentiles. The middle hinge corresponds to the median. The whiskers extend from the largest to smallest value ± 1.58 ∗ interquartile range. Samples are plotted individually, bone marrow samples (n = 3 HGBL-MYC/BCL2 and n = 1 DLBCL NOS) are indicated as square. Nonparametric Mann-Whitney U test between 2 groups was used for statistical analysis, in which P < .05 was considered significant.