T-cell functionality in patients with HGBL-MYC/BCL2, patients with DLBCL NOS, and HDs. (A-B) Percentages of total CD4⁺ (left panels) and total CD8⁺ (right panels) T-cell proliferation division index (A) and proliferation index (B) in HDs (green; n = 6), patients with HGBL-MYC/BCL2 (red; n = 20), and patients with DLBCL NOS (blue; n = 13) after 5 days of stimulation with CD3/CD28 beads. (C) Mean fluorescent intensity (MFI) of CD107a after 5 days of stimulation with CD3/CD28 beads on total CD4⁺ (left panel) and total CD8⁺ (right panel) T cells in HDs (green; n = 6), patients with HGBL-MYC/BCL2 (red; n = 23), and patients with DLBCL NOS (blue; n = 13). (D-E) Representative cytometry histograms and comparative percentages of PD-1⁺CD4⁺ (left panels) and PD-1⁺CD8⁺ (right panels) T cells for IFN-γ, TNF-α, and IL-2 and (E) MFI of CD107a on PD-1⁺CD4⁺ (left panel) and PD-1⁺CD8⁺ (right panel) in HDs (green; n = 6), patients with HGBL-MYC/BCL2 (red; n = 23), and patients with DLBCL NOS (blue; n = 12) after 4-hour stimulation with PMA/ionomysin. – represents unstimulated cells; + represents stimulated cells. Nonparametric Mann-Whitney U test was used for statistical analysis, in which P < .05 was considered significant.