Figure 6.
Deletion of IFN-1 receptor in the Townes SS mice improved the impaired BM erythropoiesis of SS mice. (A) Numbers of BFU-E colonies, CFU-E colonies, and erythroblasts in the BM of SS or SS/Ifnar1−/− mice (N = 6). (B) Quantitative analysis showing the pStat5 levels in the enriched BM erythroid cells of SS or SS/Ifnar1−/− mice. (C-D) Representative western blot and quantitative analyses of pErk levels in enriched BM erythroid cells from SS or SS/Ifnar1−/− mice. The fold changes were normalized to the pErk level from BM erythroid cells of SS mice stimulated with EPO at 1 U/mL. (E-F) Representative western blot and quantitative analyses of Stat2 levels in enriched BM erythroid cells of SS or SS/Ifnar1−/− mice. (G) Quantitative analyses of Cish mRNA levels in enriched BM erythroid cells of SS and SS/Ifnar1−/− mice. The quantitative analyses of pStat5, pErk, Stat2, and Cish were based on data from 3 biological replicates. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001.

Deletion of IFN-1 receptor in the Townes SS mice improved the impaired BM erythropoiesis of SS mice. (A) Numbers of BFU-E colonies, CFU-E colonies, and erythroblasts in the BM of SS or SS/Ifnar1−/− mice (N = 6). (B) Quantitative analysis showing the pStat5 levels in the enriched BM erythroid cells of SS or SS/Ifnar1−/− mice. (C-D) Representative western blot and quantitative analyses of pErk levels in enriched BM erythroid cells from SS or SS/Ifnar1−/− mice. The fold changes were normalized to the pErk level from BM erythroid cells of SS mice stimulated with EPO at 1 U/mL. (E-F) Representative western blot and quantitative analyses of Stat2 levels in enriched BM erythroid cells of SS or SS/Ifnar1−/− mice. (G) Quantitative analyses of Cish mRNA levels in enriched BM erythroid cells of SS and SS/Ifnar1−/− mice. The quantitative analyses of pStat5, pErk, Stat2, and Cish were based on data from 3 biological replicates. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001.

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