Clec4f⁺ Kupffer cells are an important restorative macrophage subset with increased VWF clearance activity. (A) Representative confocal microscopic images and (B) 3D rendering of confocal images of liver cryosections from TNF-challenged A/A mice, S/S mice, and S/S mice with ADAMTS13 (ATS) treatment. The sections were stained with primary antibodies to VWF, CLEC4F (Kupffer cell marker), and F4/80 (macrophage marker) (n = 5 mice per group). DAPI cell nuclear staining. (C) Quantification of the amount of VWF in CLEC4F⁺ macrophages of the 3D rendering confocal images in (B) (ie, cells stained positive for both VWF and CLEC4F). There was increased clearance of VWF in CLEC4F⁺ macrophages in the S/S mouse liver, which further increased after ATS treatment. Data represent mean ± standard deviation. ∗∗P < .01; ∗∗∗∗P < .0001, 1-way ANOVA. (D) Representative differentially expressed genes related to scavenging and anti-inflammatory functions between Kupffer cells from A/A and S/S mice challenged with TNF. For scRNA-seq, n = 2 mice per group.