Figure 1.
Generation of primitive and definitive HPCs from a FL–derived iPSC line. (A) Study design overview; iPSCs were produced from an 18 PCW healthy FL sample, and primitive and definitive HPCs were generated. (B) Schematic representation of the differentiation protocols and timeline used to generate primitive or definitive HPCs. (C) Representative flow cytometry analysis of primitive (top) and definitive (bottom) iPSC-derived HPCs. Percentage of positive cells is indicated; negative control is shown in gray. (D) Colony-forming cell frequency in primitive and definitive HPC (n = 6-9). (E) Representative images (bright field) of different progenitor-derived colonies at different magnification. Red colonies are circled. Red scale bar is 250 μm and white scale bar is 2 mm. (F) Megakaryocytic colony-forming cell frequency in primitive and definitive HPC (n = 2-4). G) Representative images (bright field) of megakaryocite colonies. Black scale bar is 200 μm.