Identification of active regulons and gene signatures in primitive and definitive iRBCs. (A) Heat map of top 10 differentially expressed regulons across Seurat erythroid clusters. (B) Heat map of a selection of differentially active regulons in erythroid clusters. The color intensity represents the average regulon activity, z-score normalized by row. (C) Violin plot illustrating regulon activity of BCL11A and SOX6 in erythroid clusters. (D) Split violin plot of BCL11A, SOX6, and KLF1 regulon activity in FL-definitive erythroid clusters. Definitive iRBCs are shown in green and FL sample is shown in blue. Adjusted P-values are calculated using Wilcoxon rank sum test with Bonferroni correction (§Padj < 1e-4, #Padj < 1e-8). (E) GSEA analysis of FL and definitive and primitive-derived RBC. Normalized enrichment scores of significant pathways (Padj < .01) are shown.
