Modulation of platelet reactivity by suppression of translation in circulating platelets. WT mice were dosed by tail vein injection with vehicle (saline, volume per volume), puromycin (20 mg/kg), or cycloheximide (120 mg/kg), 18 hours and again 4 hours before platelet collection. (A-D) Platelets from treated mice were subject to agonist stimulation as indicated in the presence of fluorophore-conjugated antibodies against activated α_IIbβ₃ integrin (Jon/A) and Cd62p, and measured by flow cytometry; n = 9. P < .03 compared with vehicle control except if indicated otherwise; other P values are shown compared with vehicle control; n.s., not significant compared with vehicle control; MFI, mean fluorescence intensity, shown ± standard error of the mean. Vehicle, green circles and green lines; puromycin, red triangles and red lines; cycloheximide, blue squares and blue lines.