Translation inhibition in vivo suppresses murine platelet ex vivo aggregation by inhibition of thromboxane generation. (A-C) Platelet aggregation in response to agonists as indicated. In panel C, platelets were pretreated with vehicle, apyrase (1 U/mL), and/or aspirin (1 mM) for 30 minutes before stimulation with 0.0025 U/mL thrombin; n = 3; ∗P < .01, ∗∗P < .03. AUC, area under the curve. (D) Washed platelets (2 × 10⁸) from vehicle- or puromycin-treated mice were suspended in 0.4 mL of buffer and stimulated with 10 μM ADP for 3 minutes, and thromboxane B2 in platelet-depleted releasates was measured as described in “Methods”; n = 5. Vehicle, green lines or bars; puromycin, red lines or bars.