Inhibition of translation in circulating platelets delays but does not compromise hemostasis in mice. (A) Time to initial stoppage of bleeding after 1-mm diameter tail amputation in C57Bl/6J mice subject to 2 doses of either vehicle or puromycin as in Figure 3. Data are replotted as box and whisker plots to the right. (B) Time to hemostasis after rebleeds in the same mice as in panel A. The experiment was stopped at 1200 seconds; n = 9. P < .28 (not significant) for final hemostasis (log-rank test). (C-H) Bleeding times and clot features were monitored under microscopic observation in C57Bl/6J mice treated as earlier, after 150-μm puncture to the jugular vein; n = 10. (C) Time to hemostasis. The experiment was stopped at 300 seconds. (D) Replots of data from panel C. (E) Representative micrographs of intraluminal sides of injury sites, resected after the experimental end point, showing platelet volumes (Cd41, purple), Cd62p (P-selectin, green), and fibrin (blue); collagen is shown in gray. (F) Cd41 volumes. (G) Cd62p mean intensities. (H) Fibrin mean intensities. (C-H) no significant differences were observed. Vehicle, green closed circles and solid green lines or light green or green bars; puromycin, red open circles and dotted red lines or red bars.